6-82 CELL-DYN 3000 System Operator’s Manual9140240E — May 1995Calibration Chapter 610. Press [RETURN] followed by [MAIN] to return to the MAINMENU Screen.Determining the Closed Mode Mean1. From the MAIN MENU Screen, press [RUN].2. Press [CHANGE SAMPLER] to select the Closed mode.3. Press [SPECIMEN TYPE].4. Use the arrow keys on the keyboard to move the cursor to an emptyQC file and type “CLOSED MEAN” in the field.5. Press the Enter key on the keyboard to save the name. Use the UpArrow key on the keyboard to move the cursor back to the“CLOSED MEAN” file.6. Press [QC SPECIMEN] to return to the RUN Screen.7. Run each sample one time into the “CLOSED MEAN” QC file.8. Press [MAIN] followed by [QC LOGS]. With the cursor at"CLOSED MEAN" file, press [VIEW QC LOG].9. Press [PRINT QC LOG] to obtain a printout of the data.10. Press [RETURN] followed by [MAIN] to return to the MAINMENU Screen.Difference Calculation1. Use the mean value for each parameter from the “OPEN MEAN”and “CLOSED MEAN” file printouts for the calculations.2. Enter the information from step 1 on the Manual Mode to ModeCalibration worksheet and calculate the Mode to Mode Calibrationbias for each parameter as follows:Closed Mode Mean - Open Mode Mean x 100 = % DifferenceOpen Mode Mean3. Enter the % difference for each parameter in the Mode to ModeCalibration Criteria chart on the worksheet. (This worksheet maybe duplicated as needed.) The Calibration Criteria are shown inTable 6.8.NOTE: Delete the sign of the factor % difference beforeentering it on the chart.4. To determine which parameters require calibration, continue withthe next section.