Chapter 2 Design the Relative Standard Curve ExperimentSet Up the Run Method35Applied Biosystems 7500/7500 Fast Real-Time PCR System Getting Started Guide for Relative Standard Curveand Comparative CT ExperimentsNotesSet Up the Run MethodIn the Run Method screen, review the reaction volume and the thermal profile for thedefault run method. If needed, you can edit the default run method or replace it with onefrom the Run Method library.About theExampleExperimentIn the relative standard curve example experiment, the default run method is usedwithout edits.Review the RunMethod Screen1. In the Run Method screen, select either the Graphical View tab (default) orTabular View tab.2. Make sure the Reaction Volume Per Well field displays 50 μL.3. Make sure the thermal profile displays the holding and cycling stages shown below.If necessary, click a temperature or time setting, then change it.4. Click Next.213
