abi 7500 Getting Started Manual

Contents

Table Of Contents
Table Of Contents
Table Of Contents
Table Of Contents
Chapter 1 Get Started
About the 7500/7500 Fast System
Supported Consumables
How to Use This Guide
About the Example Experiments
Example Experiment Workflow
Chapter 2 Design the Relative Standard Curve Experiment
Create a New Experiment
Define the Experiment Properties
Define the Methods and Materials
Set Up the Targets
Set Up the Standards
Set Up the Samples
Set Up the Relative Quantitation Settings
Set Up the Run Method
Review the Reaction Setup
Order Materials for the Experiment
Finish the Design Wizard
Chapter 3 Prepare the Relative Standard Curve Reactions
Prepare the Template
Prepare the Sample Dilutions
Prepare the Standard Dilution Series
Prepare the Reaction Mix
Prepare the Reaction Plate
Chapter 4 Run the Relative Standard Curve Experiment
Prepare for the Run
Enable the Notification Settings (Optional)
Start the Run
Monitor the Run
Unload the Instrument
Chapter 5 Analyze the Relative Standard Curve Experiment
Section 5.1: Review Results
Analyze the Experiment
View the Standard Curve
View the Amplification Plot
View the Gene Expression Plot and Well Table
Publish the Data
Section 5.2: Troubleshoot (If Needed)
View the Analysis Settings
View the QC Summary
Omit Wells from the Analysis
View the Multicomponent Plot
View the Raw Data Plot
Chapter Overview
Run the Experiment
Section 9.1: Review Results
Section 9.2: Troubleshoot (If Needed)
Section 9.3: Perform a Study of Multiple Experiments
Section Overview
Design a Study
Analyze the Study
Appendix A Alternate Experiment Workflows
QuickStart Workflow
Template Workflow
Export/Import Workflow
Bibliography
Glossary
Index

abi 7500 Getting Started Manual Manual pdf 176 page image

Chapter 8 Run the Comparative CT ExperimentChapter OverviewApplied Biosystems 7500/7500 Fast Real-Time PCR System Getting Started Guide for Relative Standard Curveand Comparative CT Experiments152NotesChapter OverviewThis chapter explains how to perform a run on the Applied Biosystems 7500/7500 FastReal-Time PCR Systems.ExampleExperimentWorkflow Design the Experiment (Chapter 6)Start Comparative CT (∆∆C T) ExperimentPrepare the Experiment (Chapter 7)Analyze the Experiment (Chapter 9)End ExperimentRun the Experiment (Chapter 8)1. Prepare for the run.2. Enable the notification settings (Optional).3. Start the run.4. Monitor the run.5. Unload the instrument.

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