Chapter 5 Analyze the Relative Standard Curve ExperimentView the Multicomponent Plot107Applied Biosystems 7500/7500 Fast Real-Time PCR System Getting Started Guide for Relative Standard Curveand Comparative CT ExperimentsNotesView the Multicomponent PlotThe Multicomponent Plot screen displays the complete spectral contribution of each dyein a selected well over the duration of the PCR run.About theExampleExperimentIn the example experiment, you review the Multicomponent Plot screen for:• ROX™ dye (passive reference)• FAM ™ dye (reporter)• Spikes, dips, and/or sudden changes• Amplification in the negative control wellsView the Plot 1. In the navigation pane, select Analysis Multicomponent Plot.Note: If no data are displayed, click Analyze.2. Display the unknown and standard wells one at a time in the Multicomponent Plotscreen:a. Select the View Plate Layout tab.b. Select one well in the plate layout; the well is shown in the MulticomponentPlot screen.Note: If you select multiple wells, the Multicomponent Plot screen displays thedata for all selected wells simultaneously.3. In the Plot Color drop-down list, select Dye.4. Click (Show a legend for the plot).Note: This is a toggle button. When the legend is displayed, the button changes toHide the plot legend.5. View the FAM dye signal. In the example experiment, the FAM dye signal increasesthroughout the PCR process, which indicates normal amplification.
