Chapter 9 Analyze the Comparative C T ExperimentView the Gene Expression Plot and Well Table165Applied Biosystems 7500/7500 Fast Real-Time PCR System Getting Started Guide for Relative Standard Curveand Comparative CT ExperimentsNotes5. View the Well Table:a. In the navigation pane, select Analysis Amplification Plot, then selectthe View Well Table tab.b. In the Group By drop-down list, select Replicate.c. Look at the CT SD column to evaluate the precision of the replicate groups. Inthe example experiment, there are no outliers.Note: To show/hide columns in the Well Table, select/deselect the column name in theShow in Table drop-down list.AnalysisGuidelinesWhen you analyze your own comparative CT experiment, look for:• Differences in gene expression (as a fold change) relative to the reference sample.• Standard deviation in the replicate groups (CT SD values). If needed, omit outliers(see “Omit Wells from the Analysis” on page 181).For MoreInformationFor more information on the Gene Expression Plot screen or Well Table, open the7500 Software Help by clicking or pressing F1.5a5b5c
