Page 147025 Instruction Manual (Rev. 2)B. Attach a clean, freely moving 10ml (or 30ml) glass syringe via a short (5cm)length of tubing to the stem of a “Y” connector. Adjust the plunger so that 1-2ml (or 3-6 ml) of air is in the syringe, before attaching the tube.Figure 8 “Checking Stroke Volume”C. Attach the two arms of a “Y” to the “room air” and “to animal” ports of the valveblock. Use short lengths of tubing. This will establish a closed system betweenthe pump cylinder and the syringe, so that the air will move back and forth frompump to syringe.D. Set the speed at about 30 S.P.M. and observe the plunger excursion as itmoves back and forth. Hold the syringe horizontally to minimize gravity effecton the plunger travel. The limits of its travel will indicate the actual amount ofair being supplied by the pump at each stroke.To check stroke to stroke volume, use a freely moving 50 ml glass syringe andconnect it with a short length of Tygon® or rubber tubing to the “to animal” port.With the piston in the max. stroke position, run the pump at about 40 S.P.M.and observe how many strokes it takes to get 50 ml. On the 5ml setting, for in-stance, it should take 10 strokes.Figure 9 “Checking Stroke to Stroke Volume”Should the foregoing test show an error in the volume output of the pump, the mostprobable cause is air leakage, see paragraph 7.1-Air Leakage.6.2 FrequencyTo check the frequency (strokes per minute), use a stopwatch. Run the pump at 60S.P.M., i.e., one stroke per second. Count 10 piston strokes then stop the watch.