Zeiss Colibri 7 Operating Manual

Also see for Colibri 7: Quick reference guide

Zeiss Colibri 7 Operating Manual Manual pdf 28 page image

OPERATIONZEISS Controlling the Colibri 7 solid-state light source Colibri 728 423052-7344-001 02/2017Image acquisition in "Continuous" mode in the "Locate" tabContinuous is the default setting for Colibri 7 in the ZEN 2.3 (blue edition) control software.When acquiring images with a camera mounted to the microscope system, the corresponding LEDs mustbe manually switched on in the Locate tab and switched off again after acquisition.• Switch on all required LEDs by clicking the corresponding checkbox and set the desired brightness.• Use the Live, Continuous or Snap buttons to acquire images (see Fig. 16).• Switch the LEDs off by clicking with the mouse.Image acquisition in "Gated" mode in the"Locate" tabGated allows the direct control of LEDs via thetrigger output of the camera.The active LED will be switched on or off with theadjusted brightness in parallel to camera exposure.The bleaching impact on the probe will be reducedby synchronizing the exposure time of Colibri 7with that of the camera.• Connect the trigger output of the camera (e.g.Axiocam 506) using the trigger cable (accessoryfor Axiocam, order number 426557-0001-000,see operating manual Axiocam) with theIncrement outlet socket on Colibri 7 (see(Fig. 4/1).• In the Locate tab, expand the Camera tool byclicking on the expander (see Fig. 18).• Activate the checkboxes Enable for Snap andEnable for Live in Trigger Control -> TriggerOut (see Fig. 18)• Activate the Gated mode in the Colibri 7control menu (see Fig. 17).• Select the LEDs by clicking the correspondingcheckbox and set the desired brightness.The LEDs will only be switched on or offautomatically during image acquisition.• Open the reflected light shutter of themicroscope.• Use the Live, Continuous or Snap buttons toacquire images.Fig. 18 "Locate" tab – "Camera" tool