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RBC Bioscience MagCore HF48 User Manual
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35www.rbcbioscience.comMagCore® Genomic DNA FFPE One-Step KitFor extraction of total DNA from formalin-fixed paraffin-embedded (FFPE) tissue by usingMagCore® System.Cartridge Code 405Cat. No. MGF-01 // MGF-03Kit ContentsCheck that the following parts are included in addition to the main unit:Cartridge Contents :Storage and Stability :vvv1. This kit should be stored at room temperature.2. Proteinase K should be stored at -20°C when mixing with PK Storage Buffer.3. Shelf life 12 months.Cat.No. MGF-01 Contents:Pre-filled Cartridge Reagent..............................................................................36 pcs.Pipet Tip plus Holder Set......................................................................................72sets.Elution Tube..............................................................................................................36 pcs.Sula Oil (25 ml)............................................................................................................1 pcs.Proteinase K(11mg).................................................................................................1 pcs.PK Storage Buffer......................................................................................................1 pcs.Thermostable cap ................................................................................................36 pcs.Cat.No. MGF-03 Contents:Pre-filled Cartridge Reagent..............................................................................72 pcs.Pipet Tip plus Holder Set...................................................................................150 sets.Elution Tube...............................................................................................................75 pcs.Sula Oil (50ml)............................................................................................................1 pcs.Proteinase K(11mg).................................................................................................2 pcs.PK Storage Buffer.......................................................................................................2 pcs.Thermostable cap .................................................................................................75 pcs.GT buffer 500μlMagnetic beadsSeparation Well 2Magnetic beadsSeparation Well 1Lysis Buffer 500μlBinding Buffer 1000μlBeads Mixture 500μlWash 1 Buffer 1000μlWash 2 Buffer 1000μlddH2O 1000μlElution Buffer 1000μlEmptyEmptyHeat block Well 2Heat block Well 1DescriptionMagCore® Genomic DNA FFPE One-Step Kit is designed for purification of total DNA from FFPE tissues by usingMagCore® instruments. It features the method, One-Step Heating, to melt paraffin and lyse tissue samples at thesame time without harmful reagents involved such as xylene. Two protocols are designed and optimized for differentsizes of tissues: 2 hrs for small samples/ 16 hrs for large samples (Please see ‘’important notes’’). DNA will be extractedfast and economically based on the cellulose coated magnetic bead technology.ApplicationsUse magnetic-particle technology to purify genomic DNA from FFPE tissue. The purified genomic DNA can be directlyused for downstream application such as PCR, real-time PCR, restriction enzyme digestion, southern blotting…etc.Introduction of each MagCore® Nucleic Acid Extraction kit |
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