Hanna Instruments HI 95724C Instruction Manual

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Hanna Instruments HI 95724C Instruction Manual Manual pdf 6 page image

6Absorption of Light is a typical phenomenon of interaction between electromagnetic radiation andmatter. When a light beam crosses a substance, some of the radiation may be absorbed byatoms, molecules or crystal lattices.If pure absorption occurs, the fraction of light absorbed depends both on the optical path lengththrough the matter and on the physical-chemical characteristics of the substance according to theLambert-Beer Law:-logI/ Io ελ c dorA ελ c dWhere:-logI/ Io = Absorbance (A)Io = intensity of incident light beamI = intensity of light beam after absorptionελ = molar extinction coefficient at wavelength λc = molar concentration of the substanced = optical path through the substanceTherefore, the concentration "c" can be calculated from the absorbance of the substance as theother factors are known.Photometric chemical analysis is based on the possibility to develop an absorbing compoundfrom a specific chemical reaction between sample and reagents. Given that the absorption of acompound strictly depends on the wavelength of the incident light beam, a narrow spectralbandwidth should be selected as well as a proper central wavelength to optimize measurements.The optical system of Hanna's HI 95 series colorimeters is based on special subminiaturetungsten lamps and narrow-band interference filters to guarantee both high performance andreliable results.PRINCIPLE OF OPERATIONBlock diagram (optical layout)19REAGENT SETSHI 93701-F 300 tests Free ChlorineHI 93701-T 300 tests Free and 100 test Total ChlorineHI 93711-D3 DPD3 reagent for 200 testsOTHER ACCESSORIESHI 95724-11 CAL CHECK™ Standard Cuvets (1 set)HI 710009 Blue rubber bootHI 710010 Orange rubber bootHI 721310 9V battery (10 pcs)HI 731318 Tissue for wiping cuvets (4 pcs)HI 731331 Glass cuvets (4 pcs)HI 731335 Caps for cuvets (4 pcs)HI 93703-50 Cuvets cleaning solution (230 mL).ACCESSORIES