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HP D300 Operating Manual
Also see for Compaq d300 Series: Reference guide
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HP D300 Digital Dispenser 264. Click OK. All dispensing for the current fluid will occur. In the main operating window, only thewells for the current fluid are shown, and each well is covered with an X while it is beingdispensed and then turns black when the dispensing for that well is complete.5. Repeat steps 1 through 4 to load and dispense each fluid in the plate configuration. Fluidloading can occur during the dispense operation; you do not have to wait until the currentDispensehead has finished. Just remember to click on OK as each fluid is loaded in itsDispensehead, or to click on OK All when all Dispenseheads of the current Cassette areloaded, as shown in Figure 4-26.6. If loading all Dispenseheads simultaneously with a multichannel pipette or individually prior tostarting the dispense, then after all Dispenseheads of the current Cassette are loaded, click onOK All as shown in Figure 4-26.Figure 4-26 Load fluid dialog box showing OK All button7. After the fluids in the currently loaded cassette are dispensed into plate 1, then dependingupon the configuration, you will be asked to either load a new cassette or a new plate. Readthe prompt dialog box carefully to determine the next step.WARNING: For multiplate configurations, the D300 software first minimizes the use ofdispenseheads, so that fluids which are used on multiple plates are dispensed into all ofthose plates as long as the total dispense volume for that fluid does not exceed 10uL. Thisdispensehead conservation can result in a non-intuitive plate loading order. Be careful toload the correct well plate as prompted by the software! Minimizing the number ofdispenseheads used may sometimes result in more plate swaps than expected.8. If you are prompted to load the next plate in the configuration, you will see a prompt similar toFigure 4-18, where you can enter or scan a name for the plate. If the plate has been loadedpreviously, you will be prompted with the plate name you previously entered or scanned in forthe plate, along with the plate number. |
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